The role of the poly(A)-specific ribonuclease (PARN) in cellular RNA metabolism
Alexandra studied biochemistry at the Martin-Luther-University Halle-Wittenberg. As a PhD student she investigates the role of the poly(A) specific ribonuclease (PARN) in cellular RNA metabolism.
In vitro studies showed that PARN removes the poly(A) tail of mRNAs which led to the conclusion that it has a function in mRNA decay. However, a closer look at the in vivo functions showed no role in general mRNA decay. In somatic cells PARN is located in the nucleus, especially in nucleoli where it functions in the processing of H/ACA box snoRNAs. This led us to the question if PARN plays a role in RNA processing rather than in mRNA decay.
We are developing a new method that theoretically can detect newly transcribed RNAs and also their 3’ ends. In the future, we plan to use this technique to identify new PARN substrates and hope to get a clearer understanding of the function of PARN.
Supervisor: Prof. Wahle
Phone: +49-(0)345 55 24949