Expression and function of the non-coding WISP1 transcript (lncWISP1) in fibrosis
Mirjana obtained a master degree in molecular biology at the University of Zagreb, Croatia. She works as a PhD student at the Julius-Bernstein Institute of Physiology since December 2014.
As part of the B1 project, her research will focus on a) the effect of lncWISP1 overexpression on microRNA (miR) and gene expression and b) interaction between lncWISP1 and proteins.
The alternative WISP1 transcript, lncWISP1, leads to increased formation of extracellular matrix (ECM) components such as collagens. This finding suggests a role of the WISP1 locus in fibrosis. To determine the effect of lncWISP1 overexpression on cellular phenotype and extracellular matrix formation, in particular collagen I, III, IV and fibronectin, Mirjana will use e.g. digital droplet PCR (ddPCR) and disease-specific gene arrays (focusing on genes related to fibrosis and/or epithelial-to-mesenchymal transition).
Supervisors: Prof. Dr. Michael Gekle and PD Dr. Gerald Schwerdt