Junior Research Group “RNA biology and pathogenesis”

Head: Jun.-Prof. Dr. Tony Gutschner

Regulation of gene expression is one of the most fascinating and most complex tasks of the cell. The research projects in our lab aim to unravel the molecular function and regulatory principles applied by non-coding RNAs (ncRNAs) and RNA-binding proteins (RBPs) in cancer cells. To achieve this goal, we will develop novel genome engineering and gene targeting strategies, which we combine with classical cell biology and biochemical approaches to dissect molecular pathways and interaction networks of these important regulators in the context of tumor biology. Ultimately, we want to identify vulnerabilities in human cancers that display an unbalanced expression of ncRNAs and RBPs leveraging unbiased genetic screening tools (e.g. CRISPR/Cas9). These efforts might reveal novel therapeutic targets and treatment options. 


Selected publications:

Gutschner T. et al. (2016). Post-translational Regulation of Cas9 during G1 Enhances Homology-Directed Repair. Cell Rep., 14(6), 1555-1566. 

Gutschner T. et al. (2014). Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) is an important protumorigenic factor in hepatocellular carcinoma. Hepatology, 59(5), 1900-11.

Gutschner T. et al. (2013). The non-coding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells. Cancer Res., 73(3), 1180-1189.

Gutschner T. et al. (2011). Non-coding RNA gene silencing through genomic integration of RNA destabilizing elements using zinc finger nucleases. Genome Res., 21(11), 1944-1954.