Microscopy and Image Acquisition

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  • High-throughput live cell analysis system for long-term experiments
  • Bright field, phase contrast and fluorescence images
  • Objectives 4x, 10x and 20x
  • Existing analysis options for: growth and cell toxicity assays, migration/scratch, spheroids, T-cell assays, angiogenesis, chemotaxis and neuronal differentiation
  • It is possible to carry out necessary cell culture steps on site
  • Laser: pulsed white light laser (470 nm - 670 nm), diode (405 nm), argon (458 nm, 476 nm, 488 nm & 496 nm)
  • Tandem scanner: conventional scanner (10-1400 Hz) and resonance scanner (8MHz)
  • Detectors: 2x HyD, 2x PMT incl. time gating and photon counting
  • Objectives: 20x - 60x with different immersion (dry, oil, glycerin and water incl. water pump)
  • Ludin cube incubation system for live cell analysis
  • Automated high content screening software (Leica HCS A)
  • HyVolution2
  • Cuda workstation and off-station including navigator, 3D visualization and Hyugens deconvolution
  • Laser: Pulsed white light laser (470 nm - 670 nm) as well as diode (405 nm), argon (458 nm, 476 nm, 488 nm & 496 nm), DPSS561 (561 nm) and HeNe633 (633 nm)
  • Tandem scanner: conventional scanner (10-1400 Hz) and resonance scanner (8MHz)
  • Detectors: 2x HyD and 3x PMT incl. time gating and photon counting
  • automated high content screening software (Leica HCS A) with DFC 360 FX camera (40 MHz)
  • Ludin cube incubation system for live cell analysis
  • FRET and FRAP software applications
  • Objectives: 20x - 100x with different immersion media (oil, dry, water)
  • 3D deconvolution and colocalization application
  • X/Y/Z motorized inverted epi-fluorescence microscope with 3D imaging option
  • Multi-positioning and stitching
  • Perfect Focus System (PFS)
  • Pecon-liveCell incubation chamber with temperature and CO2 control
  • 12 MP color camera (cooled)
  • X-Cite illumination with adjustable intensity from UV to infrared
  • Available objectives: 4x, 10x, 20x and 63x Plan-Apo, 100x TIRF
  • Available filters (motorized): DAPI, CFP, GFP, YFP, RFP, Cy5, Cy7
  • Deconvolution and real-time deconvolution equipment for noise reduction and high resolution
  • Z-Motorized Inverted Epi-Fluorescence microscope with 3D imaging option
  • DS-1QM camera with high sensitivity, which enables recordings even in the infrared range with high resolution and high recording speeds (15 frames / sec).
  • 5 MP color camera (cooled)
  • X-Cite illumination with adjustable intensity from UV to infrared
  • Available objectives: 4x, 10x, 20x and 63x plan apo, 100x TIRF
  • Available filters (motorized): DAPI, CFP, GFP, YFP, RFP, Cy5, Cy7
  • Deconvolution and real-time deconvolution equipment for noise reduction and high resolution
  • Bioscope Catalyst combines atomic force and fluorescence microscopy (Leica System)
  • Atomic force measurements can be combined with optical data
  • System and application details can be found here
  • The Core Facility Imaging only offers experienced users access to this special system

This device is located at the Medical Campus Steintor (Grosse Steinstr. 52) - Department for Anatomy and Cell Biology.

 

Contact:

Dr. rer. nat. Tim Hohmann (Tel.:0345 557 1316)

Prof. Dr. med. Faramarz Dehghani (Tel.:0345 557 1707)

  • Laser:405, 488, 561, 635 nm
  • Scanner: 400-800 Hz
  • Objektives: 10, 20, 40, 63x mit Luft-, Wasser- oder Öl-Immersion
  • Software: LasX with basic function for image post-processing (segmentation, noise filter, morphological transformations, etc.)
  • Suitable for live cell microscopy (temperature and CO2 regulation)
  • Possibility of emission wavelength resolved imaging

This device is located at the Medical Campus Steintor (Grosse Steinstr. 52) - Department for Anatomy and Cell Biology.

 

Contact:

Dr. rer. nat. Tim Hohmann (Tel.:0345 557 1316)

Prof. Dr. med. Faramarz Dehghani (Tel.:0345 557 1707)