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Research Projects and Methods

We are a research group working in the area of tumor research at the University at Halle (Saale). Focus of investigations is on clarification of the mechanisms underlying cellular radiation sensitivity of human tumors (especially radiation resistance by hypoxia), therapeutic interventions against molecular target genes in order to improve radiotherapy of tumors, the trial of new chemotherapeutic substances in combination with radiotherapy, and the identification and characterization of diagnostic, prognostic and therapeutic markers in tumors (like glioblastomas, head and neck tumors, bronchial carcinomas and soft tissue sarcomas).

Our work was/is funded by several institutions (Deutsche Krebshilfe, Deutsche Forschungsgemeinschaft, Sanderstiftung, Wilhelm-Roux program).

Current research projects:

  • Targeting of carboanhydrase IX in combination with irradiation in human tumor cells 
      • Impact of betulinic acid and derivatives together with irradiation on human tumors
          • Modulation of radiation sensitivity of human gliomas by IDH1
          • Expression analyses of hypoxia and tumor markers or miRNAs in head and neck tumor patients and patients with bronchial carcinomas or glioblastomas before and during radiotherapy
          • MiRNA expression analysis as a function of irradiation and tumor hypoxia in human tumor cell lines
          • Examination of potential EGFR-Hif1α-survivin-pathway

             

            Material und methods:

            • Cell culture, tumoral tissue, plasma and serum
               
            • Irradiation by linear accelerator

            • Knock down / overexpression by siRNA / plasmid transfection

            • Colony formation, SRB-assay, growth analyses, spheroid formation assay, LDH- assay, radiation sensitivity

            • Migration-, apoptosis- und cell cycle examinations

            • γH2AX assay, ROS-assay, fluorescence microscopy, intracellular pH-measurement

            • RT-PCR (real-time PCR, multiplex PCR)

            • Westernblot, ELISA, IHC 

            Martin Luther University at Halle (Saale)
            University Hospital - Department of Radiation Oncology

              Chairman:
              Prof. Dr. med. Dirk Vordermark
              Secretary:
              Stephanie Fechner
              Phone: ++49 345 557 4310

            Postal Address: 
            Universitätsklinikum Halle (Saale)
            Klinik und Poliklinik für Strahlentherapie
            Ernst-Grube-Straße 40
            D-06120 Halle (Saale)
            Germany
            e-Mail:

            Strahlentherapie@uk-halle.de