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Welcome to the AG Hollemann - Medical Molecular Biology

 

 Prof. Dr. Thomas Hollemann

 

   

My primary expertise is the identification and functional analysis of regulators important for early neural development in Xenopus. In the past we focused mainly on transcription factors and how they add to the formation of the visual system. Recently we extended our research to understand how these DNA-binding transcription factors are regulated on protein level by E3-ubiquitin ligases of the Trim-family e.g. leading to degradation of the transcription factors by the proteasome. Meanwhile, we established more unbiased approaches (enzyme activity or interaction based) using protein chips and label-free proteomics to identify proteins that are modified by E3-ubiquitin ligases of interest.

 

Thus, we extended our panel of classical amphibian techniques from whole-mount in situ (RNA & immuno), histology, explantation and transplantation of embryonic tissues, stem cell (animal caps), (RNA & morpholino injections; live imaging of larval development), cell culture of mouse and human cell lines incl. gain- and loss-of-function approaches (transfection & Crisp/Cas), imaging, FACS. More specialized methods include: Nanostring (quantification of RNAs without amplification of the probes, protein (co)-ip, protein-arrays (ubiquitination assay on a chip), proteomics of cell cultures and embryos in collaboration with our core-facility (Orbitrap Exploris 480 with FAIMS). 

 

 

Most significant publications 

1.      Grunewald S, Marillonnet S, Hause G, Haferkamp I, Neuhaus E, Veß A, Hollemann T, Vogt T. (2020) The tapetal major facilitator NPF2.8 is required for accumulation of flavonol glycosides on the pollen surface of Arabidopsis thaliana. Plant Cell. 32(5):1727-1748. doi: 10.1105/tpc.19.00801.

2.      Liu H, Ding J, Köhnlein K, Urban  N, Ori  A , Villavicencio-Lorini P, Walentek P, Klotz LO, Hollemann  T, Pfirrmann  T. (2019). The GID ubiquitin ligase complex is a regulator of AMPK activity and organismal lifespan. Autophagy. 1–17. doi:10.1080/15548627.2019.1695399

3.      Pfirrmann T, Jandt E, Ranft S, Lokapally A, Neuhaus H, Perron M, Hollemann T. (2016). Hedgehog-dependent E3-ligase Midline1 regulates ubiquitin-mediated proteasomal degradation of Pax6 during visual system development. Proc Natl Acad Sci. USA 113:10103-10108.

4.      Metikala S, Neuhaus H, Hollemann T (2016). Suppression of vascular network formation by chronic hypoxia and prolyl-hydroxylase 2 (phd2) deficiency during vertebrate development. Angiogenesis 19:119-131.

5.      Pfirrmann T, Emmerich D, Ruokonen P, Quandt D, Buchen R, Fischer-Zirnsak B, Hecht J, Krawitz P, Meyer P, Klopocki E, Stricker S, Lausch E, Seliger B, Hollemann T, Reinhard T, Auw-Haedrich C, Zabel B, Hoffmann K, Villavicencio-Lorini P. (2015b). Molecular mechanism of CHRDL1-mediated X-linked megalocornea in humans and in Xenopus model. Hum. Mol. Genet. 24:3119-3132.

6.      Pfirrmann T, Villavicencio-Lorini P, Subudhi AK, Menssen R, Wolf DH, Hollemann T. (2015a) RMND5 from Xenopus laevis Is an E3 Ubiquitin-Ligase and Functions in Early Embryonic Forebrain Development. PLoS One 10:e0120342.

7.      Pfirrmann T, Lokapally A, Andréasson C, Ljungdahl P, Hollemann T. (2013). SOMA: a single oligonucleotide mutagenesis and cloning approach. PLoS One 8:e64870. 

8.      Neuhaus H, Argia A, Hollemann T. (2010). Xenopus er71 is involved in vascular development. Dev. Dyn. 239:3436-3445.

9.      Wu HY, Perron M, Hollemann T. (2009). The role of Xenopus Rx-L in photoreceptor cell determination. Dev Biol. 327:352-365. 

10.   Hollemann T, Chen Y, Grunz H, Pieler T. (1998) Regionalized metabolic activity establishes boundaries of retinoic acid signalling. EMBO J. 17:7361-72.

11.   Hollemann T, Bellefroid E, Pieler T. (1998) The Xenopus homologue of the Drosophila gene tailless has a function in early eye development. Development 125:2425-32.

12.   Bellefroid EJ, Bourguignon C, Hollemann T, Ma Q, Anderson DJ, Kintner C, Pieler T. (1996) X-MyT1, a Xenopus C2HC-type zinc finger protein with a regulatory function in neuronal differentiation. Cell 87:1191-202.

 

Direktor:
Prof. Dr. Guido Posern

Abt. Hollemann: 
Telefon: (0345) 557-3868 
Email:     Email

Hausanschrift:
Institut für Physiologische Chemie
Hollystrasse 1
06114 Halle (Saale)

Assistenz der Institutsleitung:
Ines Knipping
Telefon: (0345) 557-3812 
Fax:        (0345) 557-3811
Email:    Email