The vast majority of all eukaryotic mRNA-precursors are 3' processed by cleavage and polyadenylation. The Cleavage and Polyadenylation Specificity Factor (CPSF) is a multi-protein complex, crucial for both 3' processing reactions.
The first components of mammalian CPSF have been purified and characterised in the early 90's. However, reconstitution attempts with recombinant proteins failed so far, so the actual composition of mammalian CPSF still remains elusive.
Using a baculoviral expression system optimized for expression of multi-protein complexes, we are making another attempt at reconstitution. We want to express, purify and characterise all known, as well as putative subunits of mammalian CPSF and identify the proteins that provide the functions of CPSF in cleavage and polyadenylation of eukaryotic mRNA-precursors.
Supervisor: Prof. Wahle
Phone: +49 (0)345-55-24952