![Sequenziergerät](/fileadmin/_processed_/2/5/csm_Sequenzierger%C3%A4t_print__5__44c58e9186.jpg)
The Illumina NextSeq 1000 offers diverse applications for high-throughput DNA and RNA-sequencing.
Further information can be found on the Core Facility Imaging (CFI) website.
- Transcriptome analysis using RNA-seq (mRNA, lncRNA, medium-sized RNA, smallRNA)
- Translation control using Ribo-seq
- Binding analyzes using ChIP- and CLIP-seq
- Genome analysis using Whole Genome-seq or Exom-seq
- Oxford Nanopore DNA/RNA-seq data
- Quality control of raw data
- Removal of adapter sequences and quality trimming
- Mapping (assignment of reads to known reference genomes)
- Annotation (assignment of mapped reads to known genes)
- Determination of read coverage
- Normalization (regarding RNA composition, transcript length, differential expression etc.)
- Determination of differentially expressed genes
- Correlation of mRNA to miRNA expression (incl. target analysis)
- Determination of the expression of alternative isoforms (e.g. due to alternative splicing)
- Quantification of translation efficiency (Ribo-seq)
- Analysis of binding profiles (ChIP- and CLIP-seq)
- Determination of effector pathways using gene annotation enrichment analysis (GAEA) and gene set enrichment analysis (GSEA)
- Metagene analyses, inclusion of "public data" (e.g. TCGA) and further analyzes on request
![](/fileadmin/_processed_/4/d/csm_mini_51e29f49eb.png)
- Support for Oxford Nanopore sequencing
- Optional library preparation (barcoded)
- data analysis
List of fees for the evaluation and further analysis of NGS data on known genomes.
Service | Medical Faculty | MLU (not Med. Faculty) | Billing Unit (BU) |
Consultation | 30€ | 50€ | per call |
Quality control small RNA (RIN) | according to consumption | according to consumption | per chip (max. 11 samples/chip) |
Quality control RNA (RIN) | according to consumption | according to consumption | per chip (11-12 samples/chip) |
Primary Analysis | 10€ | 20€ | per 20 million reads¹ |
Secundary Analysis | 25€ | 30€ | per analysis |
¹ Per started billing unit.
Data retention for 3 months.